Continuous directed evolution of proteins with improved soluble expression
نویسندگان
چکیده
منابع مشابه
ECSTASY, an adjustable membrane-tethered/soluble protein expression system for the directed evolution of mammalian proteins.
We describe an adjustable membrane-tethered/soluble protein screening methodology termed ECSTASY (enzyme cleavable surface tethered all-purpose screening system) which combines the power of high-throughput fluorescence-activated cell sorting of membrane-tethered proteins with the flexibility of soluble assays for isolation of improved mammalian recombinant proteins. In this approach, retroviral...
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ژورنال
عنوان ژورنال: Nature Chemical Biology
سال: 2018
ISSN: 1552-4450,1552-4469
DOI: 10.1038/s41589-018-0121-5